The music was very unsuited to the scene portrayed. That was just wrong and some pretty bad sound design. One major issue, during a fight scene in the first episode, they play a pop track, complete with high-pitched female vocalist. The second set of intro and outro were much better. The outro was better though, but not amazingly so. Seeing it in the second episode, I personally don’t like it. The intro to this anime wasn’t there in the first episode. But ignore that, my recommendations for this anime are irrespective of gender. This in combination with the almost bishounen, pretty boy-like designs of some of the male characters makes me think this is a shoujo anime. There is no fan-service or ecchi I’m happy to say. I can still like an anime that looks like this, even if it looks a bit strange and most importantly I just got used to it. But after all that, this style of animation doesn’t affect the enjoy-ability of this anime that much. I guess I understand their perspective a bit better now. The eyes seem too big and unnatural or something like that. It’s anime like this that reminds me of those who are uncomfortable watching anime as the style is off-putting. I guess I should commend this rare type of character design though. There’s even a lightly shaded hand-drawn style used to good effect. They also make good use of facial expression and comic effects. It’s kinda inconsistent, since other times it looks normal. As a skinny person myself, I can assure you that such a training regimen should at least put on a little weight/bodymass. ![]() Two of the characters are supposed to be skilled in pro-wrestling and they work out for it. It’s a big different to other anime which have tried the tall and thin character style, like Code Geass where it was much more stylised and done better. When we first got a full body shot of the protagonist, I thought: wow, she’s very tall and skinny, how is she not falling over. ![]() It’s fairly original, though the faces of the characters seem familiar the most noticeably different facet of style are the bodies. The animation style however was certainly… interesting. ![]() A pleasant surprise after the last anime I watched, where even a higher resolution didn’t help too much. It’s not above average, but it’s acceptable for a 2008 anime. But even for an anime limited by its resolution, the quality is decent. The best quality it exists in seems to be 480p. Usually these types of anime don’t have a satisfying end. However, ‘high-school’ and ‘romance’ are two thing that don’t do well together, that’s just my opinion. It’s a sensible romance, suitable for most, but leans more towards shoujo. Considering the story, this is something that I’ve seen before in Kaichou wa Maid-Sama!, which I found funny and enjoyed. Special A is a 24 episode high-school (slice-of-life style) comedy romance anime.
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![]() The g7 is known for being a total gamble if you get a good panel or not with good backlighting. But I wanted to know if anyone could share their calibrated ICC/color profile which I could try on my monitor to see it that is better than the factory calibration. I know that color profiles are different for every panel and also depend on room lighting etc. I thought of buying a monitor calibrator, but those are just too expensive, and I won't have many uses for it after a few times. I tried tweaking the colors in Nvidia control panel and the monitor itself, but I can't find the sweet spot. I own a Samsung G7 32" and I just moved from a 100% RGB display to this one, and I don't really like the calibration and color profile for this monitor. SPEAKERS : CORSAIR SP2500 + FX Audio DAC-X6įull specs and setup details on my profile-page #2 MONITOR : Samsung 49" Odyssey G9 QLED (32:9, 10bit R G B, HDR1000) #1 MONITOR : Samsung 49" Odyssey CRG9 QLED (32:9, 8bit R G B, HDR1000) PSU: CORSAIR HXi Series HX1200i (1200 Watt 80-PLUS Platinum)ĬASE FANS: 4x CORSAIR SP140 R G B Elite, 2x CORSAIR 120LL R G B #2 SATA SSD : Crucial MX300 1.05TB (RAID 0 striped with 1.05TB Partition on #1 SATA SSD: MX500) #1 SATA SSD : Crucial MX500 2TB (1.05TB Partition RAID 0 striped with #2 SATA SSD: MX300) PCIe USB-A BOARD : ST Lab PCI-e 3.0 USB 3.1 (4x USB-A ports) GPU : ASUS ROG STRIX RTX 3080 Ti OC (300W optimized undervolt profile) CPU : Intel Core 4.7GHz, -0.095mV (Custom CNP-IHS + LM)ĬPU COOLER : CORSAIR Hydro Series H100x + 2x CORSAIR 120LL R G B But it is also doubtful whether the ruling elites would allow such real political participation. ![]() In this environment, the creation of political parties, founded by popular figures and called upon to collaborate in introducing reforms, could be useful. At the same time, any attempt at reform may meet with mistrust from society at large, because people ultimately distrust the government and those in power. But there is no doubt that even purely economic reforms cannot be successfully introduced without political ones.Įconomic and social reforms can meet violent opposition from corrupt officials, who see their sources of profit threatened. Unemployment was also very high, particularly among the rural population. Before they broke out, inflation was fluctuating between 20 and 30 percent, without salaries and incomes increasing to the same extent. The immediate causes of the protests were economic and social. There are still the economic problems and the old models of government, where clan politics means power is divided among its own men. However, even all this has not been enough to stabilize the situation in the short term. This allowed Toqaev to distance himself from the “Nazarbayev clan.” Besides consolidating his own power, the president managed to gain wide support from the populace, because the old elite was considered corrupt and Másimov’s elimination was seen as a blow against corruption. It is true that people linked to Nazarbayev participated in the protests. Másimov, whose background and close relations with China made him a potential rival to Toqaev, was thus neutralized without provoking any outcry from the elite who supported him. Although there was no evidence that former President Nazarbayev was in any way personally involved in these protests, his former head of government and national security chief, Kárim Másimov, was arrested on charges of high treason. The main outcome of the January 2022 protests was President Toqaev’s consolidation of power. However, he not only succeeded in suppressing the protests, but also freed himself from the dominance of the former president and all the men Nazarbayev had placed in positions of power. Before the protests broke out, Toqaev was considered a weak leader, a puppet in the hands of Nazarbayev. The losers were the former president, Nursultan Nazarbayev, and his supporters. The winners from these protests were the incumbent president, Qasym-Jomart Toqaev, and all his followers. Muslim extremists also mostly come from this social group, and in this Kazakhstan does not differ from many other countries. The main group involved, however, were the so-called mambets, young people from rural backgrounds who have moved to the big cities in search of better living conditions. It is still not yet clear which of the competing elites was the first to try to exploit them Islamist groups also tried to become involved. ![]() The protests broke out spontaneously, and then, as is almost always the case in such situations, there were several attempts to exploit them for political ends. However, that was only the trigger amidst general discontent. The immediate reason was the increase, after the government removed price controls, in the price of petroleum, which is very important in Kazakhstan as fuel for transport. Initially, the demands were purely economic. Protests erupted on January 2 in the oil- and gas-producing regions, which should be rich but are in fact among the poorest in the country. But one thing seems clear: if the reforms fail, the country will certainly not be able to return to peace. The fact that Kazakhstan’s leadership called a referendum for June 2022 can be interpreted either way. There are, however, big differences in the way the ruling elites react to protests, ranging from attempts to consolidate power through repression to introducing some reforms, even if these can sometimes be a fig leaf masking a tight grip on power. Kazakhstan is not an exception, just one more example. The tragic events of January 2022 once again revealed how the republics that emerged from the collapse of the USSR are still a long way from stability. The first drought and the first corpses is a typical situation for an opening game. And for this it is necessary to organize the production of materials and provide a sufficient number of workers, who, in turn, need to eat something and live somewhere. Beavers, exhausted by thirst, die very quickly, so it is better to stock up ahead of time – for storage, you can build special barrels that do not care about drought. The fact is that at the end of each time cycle, a drought sets in – rivers and reservoirs, from where the inhabitants take water for drinking, gradually become shallow and eventually dry up completely. Water plays a huge role in the life of the settlement. The ability to place buildings on top of each other, developing the city in a vertical plane, is another important feature of the game. There would be food (albeit the simplest, such as berries) and, most importantly, water. However, there is no particular point in maintaining them at a high level yet – beavers do their job anyway. There are other needs: communication, spirituality, comfort – more than ten items in total. Food is needed both for the banal survival of the inhabitants, and to satisfy their needs for a variety of diet – this gives positive effects, such as increased strength or increased speed of carrying objects. The story is the same with food products: wild berries, three types of seed plants and three buildings for their processing – a grill, a mill and a bakery, that’s all. A rather meager set of production chains, but you can write it off as early access. Beavers cut trees into logs, and from them they already make all other materials – boards, wooden gears and paper. Wood is valuable here – it is the main building resource for both factions. If the river becomes shallow, then windmills will take over. They do not reproduce naturally, unlike the beavers from the Tails of the World, but raise new inhabitants in incubators, and in general are more technologically advanced – they, for example, have a wood-burning engine. Iron Teeth, one of the two factions currently available, are more inclined towards metal. Only the ruins of cities, which can be found on the map and dismantled for spare parts, remind of the former greatness of people – iron will also come in handy for beavers. But for unknown reasons, humanity died out, but the little lovers of nibbling trees remained. To be precise, there is no specific goal here – entertain yourself, which also fits into the genre, in which the main thing is the process, not the result.ĭespite the fact that the main characters in Timberborn are beavers, once upon a time this world belonged to people. At the beginning we have only one building and a dozen inhabitants, the player’s task is to build a huge city inhabited by hundreds of beavers. Timberborn is a classic city-building strategy, at first glance, even too standard. ![]() Dams and dams, industrial-scale logging and landscape modification sound tempting, but is there enough content in this “early access” for an interesting and comfortable game? Treeborn ![]() This is in the Mario Kart channal that comes with the game. Also, this Mario Kart game comes equiped with the ability to use the Spot Pass feature and Street Pass (you can exchange information with people you pass on the street such as ghost data and best times and try to beat them. Main game you can change the setting, however in the balloon pop game you have a 1st person perspective and you have the choice in game of what you do (though you could incorporate both here if you so choose. This is pretty a pretty cool feature but you don't have to race this way if you don't wan't. A completely new feature to this Mario Kart game unlike any other (at least for the hand held variety) is that you can steer simply by moving the 3DS system to the left or right. I imagine that if you get 3 stars on every race from every class you'll unlock something. ![]() As well as obtaining a gold trophy in the different cups within the classes, the game rates you from 1 to 3 stars based on driving ability. You unlock character as you go and as you go the races get harder. There is also a 7 icon pick up in which you'll get 7 different weapons at the same time (red shell, green shell, banana, bomb, mushroom, etc.). There are a few new weapon pick ups as well, such as the racoon tail and the ability to shoot fireballs from the fire flower. Also there are more characters that you can play with, including your Mii (once you beat all 50CC races) and now you can float through the air as well as drive your kart under water. This time around it's in 3D! It is pretty cool. This game is a lot of fun and retains all of the elements from the origiginal adds a lot more to it as well. I still remember playing to original released on the SNES quite ofen. Well Nintendo decided to put out yet another Mario Kart. MARIO KART 7, CLASSIC RACING GAME, A LOT OF FUN!!!! A second issue we and other groups have encountered is the potential for LSD1 inhibitors to inhibit HRP. 1 This peptide is a potent inhibitor of LSD1, however, this methodology was not available at the time our manuscript was published. It is now known that the LSD1 reaction can be quenched using the 21-mer peptide in which dimethyllysine 4 is replaced by a methionine. The first issue, which is manageable, is that there is no reagent in the kit with which to quench the enzymatic reaction, and thus the timing of each step in the procedure is critical to obtaining reliable results. The radical cation is then quantitated with Amplex Red. Recombinant LSD1 and the peptide substrate are co-incubated, and the activity of the enzyme is determined by converting the LSD1 by-product H 2O 2 to a radical cation using horseradish peroxidase (HRP). The assay employs a 21-mer peptide substrate analogous to the tail found on histone 3, containing dimethylated lysine at position 4. The LSD1 assay procedures in the manuscript were conducted using a commercially available LSD1 assay kit (BPS Bioscience, San Diego, CA, kit #50106). Since the original publication of this manuscript, we have encountered problems with the assay procedure that was used to gather the data for the kinetic analysis of compound 6d. The correct graph is shown in the figure, generated as described below. However, the data obtained in the absence of inhibitor was inadvertently omitted from the figure. 4 on page 1605, a Lineweaver-Burk graph was inserted that indicated compound 6d competitively inhibited lysine-specific demethylase 1 (LSD1) with a Ki of 2.4 μM. The table below summarizes the possibilities.The Authors regret that in Fig. The other oxygens in the backbone can only act as hydrogen bond acceptors. The oxygens at 7 and 12 can act as either donors or acceptors. Unlike the backbone of a protein, RNA backbone doesn't contain any nitrogen atoms, so hydrogen bonding must involve oxygen atoms. In DNA, the oxygen at atom 12 is not present, and the first base atom is 12. The first atom of the base in RNA is atom 13. Atom numbering continues normally from there, and no "virtual atoms" are involved. Since there's no next RNA segment, the oxygen at atom 9 is not attached to a phosphate group. Foldit still identifies atoms 1 through 4 as being present, and these "virtual atoms" can even be banded.įor the last RNA segment in a chain, numbering is more straightforward. The first atom visible is atom 5, a carbon attached to the ribose ring. Atom 12 is the oxygen in the hydroxyl group, which Foldit considers to be the first "sidechain" atom, meaning it's not part of the backbone.įor the first RNA segment in a chain, the phosphate group is missing. Atom 10 is a carbon (carbon 1'), which is where the actual RNA base is attached. Atom 9 is an oxygen which joins the phosphate group of the next RNA segment. Foldit considers one of the oxygen atoms of the phosphate to belong to the ribose of the preceding segment, so only the oxygen atoms 2 through 4 are numbered.Ītom numbering continues with the ribose group. Foldit starts numbering with the phosphorous in the phosphate group. For example, the C terminal of a chain has an extra backbone atom, so it's beta carbon, the first atom of the sidechain, is 6 instead of 5.įor RNA, the backbone atoms always have the same number, regardless position in the chain. In a protein, the number of atoms in a segment is different depending on its position in the chain. The rules for numbering atoms are different in RNA, however. Just as in a protein, Foldit can identify atom numbers in RNA. The hydroxyl group is what distinguishes RNA from DNA. A hydroxyl group (oxygen and hydrogen) is attached to carbon 2'. The RNA base is attached to carbon 1' of the ribose. The carbon atoms are referred to as 1', 2', 3' and 4'. The sugar group in the RNA backbone is always a form of ribose, a pentagonal ring consisting of an oxygen atom and four carbon atoms. Each segment of RNA or DNA is linked to the next by a phosphate. A phosphate group consists of a phosphorous atom bound to four oxygen atoms. This is why "RNA" stands for "ribonucleic acid", and "DNA" stands for "deoxyribonucleic acid".īoth RNA and DNA backbone consists of alternating groups phosphates and sugars. The difference is that RNA backbone contains the sugar ribose, while DNA backbone contains the sugar deoxyribose. RNA backbone is highly similar to DNA backbone. The backbone of RNA is more complicated than the backbone of the amino acids in a protein. The blue numbers 1' to 4' refer to the carbons in the in ribose ring. [So u know this is not true ,oxygen can only make two bonds so it (oxygen)could not be top of pyramid in a tetrahedron formation.only carbon can make four bonds[Ifįile:Rna_|thumb|400px| RNA backbone showing chemical groups. ![]() With this technology you can easily record any type of media including live broadcasts, music albums, movies or. Link has been removed because of violating copyright laws. The new generation of televisions such as the 4K Stogram model can be used to record and transfer almost any digital video file including live television broadcasts, DVD material and even computer games. Use 4K Stogram on your PC, Mac or Ubuntu for free. Login with your Instagram credentials and download photos of private friends accounts.Īccess to your viewer directly from the application.Įnjoy the minimalistic interface of application. To set up and manage your notifications, you can head to Preferences, then Notifications. 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They banter comically, help the nun with groceries and remind us that the mad never leave madhouses in a tragicomic joke that Celestini repeats throughout. Thirty years later, Nicola (Celestini) and his only companion (Giorgio Tirabassi, a wonderful actor Italian cinema too often ignores) live a quiet routine in the institution. The black sheep becomes sacrificial lamb. When a tragedy occurs, Nicola is wrongfully blamed and officially locked up. ![]() To keep the “black sheep” of the family - the only member neither as crude nor violent as the rest - from giving his siblings away, Nicola is sent to live as a guest with the nun (Luisa De Santis) who runs the mental asylum. ![]() Nicola has a fertile imagination - particularly for martians - but is failing school and loves only his classmate Marinella (Wally Galdieri) while the rest of his peers mock his poverty and backward ways.ĭuring the summer, Nicola goes to help his brothers, who commit a terrible crime. On the contrary, the film works thanks to its well-written roles and great performances, especially from intense young newcomer Luigi Fedele.Īt the end of the “fabulous 1960s,” 10-year-old Nicola (Fedele) lives with his grandmother, who tends chickens and was “born old, lived old and died old.” His mother is vegetating in a mental institution, his sheepherder father (Nicola Rignanese) and two older brothers (Alessandro Marverti, Mauro Marchetti) live up in the hills. Most importantly, it doesn’t sacrifice character development. Celestini’s frequent off-camera narration doesn’t guide or illuminate events, it tells a story, flows along streams of consciousness and repeatedly tries to make sense of the senseless. But this is a true example of “auteur” cinema that melds the boundaries between the written word and classical visual storytelling. Nonetheless, the first Italian film in competition at this year’s Venice International Film Festival will divide viewers, many of whom will not appreciate the narrative-heavy style. |
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